Gummi candies and marshmallows seem to be particularly problematic foods for people who react to gelatin ( 27, 28, 57).Fewer than 10% of people with AGS react to gelatin in foods ( 57).Swallowing medications that contain gelatin, such as medications in gelatin capsules.The risks related to gelatin exposure are profoundly different depending on the route of exposure. People with alpha-gal syndrome (AGS) can react to gelatin derived from mammals, like bovine or porcine gelatin. Gelatin is used in foods, personal care products, medications and other medical products. Gelatin can also be listed on labels as hydrolyzed collagen ( 99), hydrolyzed animal protein, collagen hydrolysate, denatured collagen, and gelatina. Other names for gelatin, including trade names, are listed here and here. Some products marketed to people who avoid porcine or bovine gelatin due to religious prohibitions contain fish gelatin.Gelatin from fish or chicken doesn’t normally contain alpha-gal.Less than 1% of gelatin is derived from fish or chicken.Gelatin made from pigs is called porcine gelatin.Gelatin made from cows is called bovine gelatin.Pigs and cattle are mammals, and gelatin derived from them contains alpha-gal.Most gelatin is produced from the skin and bones of pigs and cattle.Collagen is found in the cartilage, bones, tendons, ligaments, and skin of animals. Gelatin (or gelatine) is a translucent, tasteless, soluble mixture of peptides and proteins derived from collagen. Medications and Other Medical Products–coming soon.Negative for Pork Contamination: P/N value below 1. Positive for Pork Contamination: P/N value equal or above 1.3 Allow to warm up to room temperature (18-25oC) before use. Sensitivity and SpecificityĪ study carried out using a total of 30 known samples and statistical analysis reported Sensitivity, Specificity, Positive Predictive Value and Negative Predictive Value of 99-100%. The test is able to detect as low as 0.5 mg of pork gelatin in 1.0 ml of sample diluents. The test can detect approximately 0.5% (w/w) pork in gelatin based samples. Instruction manual (product insert) (1 copy).Negative control, ready-to-use (1x10ml).Positive control, ready-to-use (1x10ml).Streptavidin HRP, ready-to-use (1x10ml).Biotin-conjugated anti-porcine meat antibody, ready-to-use (1x100ml).Sample diluents buffer, ready-to-use (1x100ml).Microplate (96-well), pre-coated with porcine meat-specific antibody (1 piece).User friendly and highly sensitive assay developed in sandwich ELISA method. Higher specificity with the use of porcine-specific monoclonal antibodies.Able to detect heat-stable protein (up to 132☌).The monoclonal antibody used also found and proven to be capable of differentiating pork gelatin from gelatin of other species like beef, lamb, chicken, duck, horse, deer and rabbit. Therefore, it is suitable for testing wide range of food products that are available in the market. It is proven that the protein can be detected even the sample is severely heated (132☌, 2 hours). The protein will not denature even it is exposed to high temperature for long time. Porcine-specific monoclonal antibody used in the assay is targeting a heat-stable protein. The assay is sensitive and specific to porcine meat fast and able to run multiple samples simultaneously simple sample preparation and only requires minimal sample volume. PorcineTrace ELISA Kit – Gelatin provides an excellent methodology to detect porcine gelatin contamination in gelatin or gelatin based food products such as marshmallow, jelly, collagen, capsule and etc. PorcineTrace ELISA Kit – Gelatin Description
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